Picture 1 of 1
Picture 1 of 1
Methods in Biotechnology by Seung-Beom Hong (English) Paperback Book
US $118.57
Condition:
2 available
Shipping:
International shipment of items may be subject to customs processing and additional charges.
Located in: Calgary, Alberta, Canada
Delivery:
Estimated between Mon, Jun 3 and Tue, Jun 11 to 43230
This item has an extended handling time and a delivery estimate greater than 15 business days.
Please allow additional time if international delivery is subject to customs processing.
Returns:
Payments:
*No Interest if paid in full in 6 months on $99+. See terms and apply now- for PayPal Credit, opens in a new window or tab
Earn up to 5x points when you use your eBay Mastercard®. Learn moreabout earning points with eBay Mastercard
Shop with confidence
Seller assumes all responsibility for this listing.
eBay item number:395295572078
Item specifics
- Condition
- ISBN-13
- 9781119156789
- Book Title
- Methods in Biotechnology
- ISBN
- 9781119156789
- Publication Year
- 2016
- Type
- Textbook
- Format
- Trade Paperback
- Language
- English
- Publication Name
- Methods in Biotechnology
- Item Height
- 0.9in
- Item Length
- 10.9in
- Publisher
- Wiley & Sons, Incorporated, John
- Item Width
- 8.4in
- Item Weight
- 36.1 Oz
- Number of Pages
- 368 Pages
About this product
Product Information
As rapid advances in biotechnology occur, there is a need for a pedagogical tool to aid current students and laboratory professionals in biotechnological methods; Methods in Biotechnology is an invaluable resource for those students and professionals. Methods in Biotechnology engages the reader by implementing an active learning approach, provided advanced study questions, as well as pre- and post-lab questions for each lab protocol. These self-directed study sections encourage the reader to not just perform experiments but to engage with the material on a higher level, utilizing critical thinking and troubleshooting skills. This text is broken into three sections based on level - Methods in Biotechnology, Advanced Methods in Biotechnology I, and Advanced Methods in Biotechnology II. Each section contains 14-22 lab exercises, with instructor notes in appendices as well as an answer guide as a part of the book companion site. This text will be an excellent resource for both students and laboratory professionals in the biotechnology field.
Product Identifiers
Publisher
Wiley & Sons, Incorporated, John
ISBN-10
1119156785
ISBN-13
9781119156789
eBay Product ID (ePID)
219408523
Product Key Features
Publication Name
Methods in Biotechnology
Format
Trade Paperback
Language
English
Publication Year
2016
Type
Textbook
Number of Pages
368 Pages
Dimensions
Item Length
10.9in
Item Height
0.9in
Item Width
8.4in
Item Weight
36.1 Oz
Additional Product Features
Lc Classification Number
Tp248.22
Table of Content
Preface vii Acknowledgements ix Introduction to biotechnology lab xi About the companion website xix Part I: Methods in Biotechnology (MB) Laboratory Exercises 1 1 MB experiment 1: Lab measurements 3 2 MB experiment 2: Use of the spectrophotometer and Beer''s law 9 3 MB experiment 3: Making solutions and buffer efficacy 15 4 MB experiment 4: Acid-base titration 19 5 MB experiment 5: Protein denaturation and precipitation 23 6 MB experiment 6: Bacterial transformation 27 7 MB experiment 7: GFP purification 33 8 MB experiment 8: SDS-PAGE analysis 37 9 MB experiment 9: DNA isolation 41 10 MB experiment 10: PCR-based Alu -human DNA typing 45 11 MB experiment 11: Restriction enzyme digestion 51 12 MB experiment 12: Agarose gel electrophoresis 53 13 MB experiment 13: Ouchterlony and ELISA immunoassays 57 14 MB experiment 14: Testing plant substances for antimicrobial activity 63 15 MB experiment 15: Peroxidase enzyme activity assay 67 Part II: Advanced Methods in Biotechnology (AMB) 1 Laboratory Exercises 71 16 AMB 1 experiment 16: Aseptic technique and culture handling 73 17 AMB 1 experiment 17: Yeast culture media preparation 77 18 AMB 1 experiment 18: Growth curve 79 19 AMB 1 experiment 19: Mini plasmid prep 83 20 AMB 1 experiment 20: Restriction digestion, purification concentration and quantification of DNA 87 21 AMB 1 experiment 21: Polymerase chain reaction (PCR) 91 22 AMB 1 experiment 22: TA blunt end SLIC, and CPEC cloning of PCR product 97 23 AMB 1 experiment 23: One-step multifragment assembly cloning 103 24 AMB 1 experiment 24: Restriction enzyme digestion and fast agarose gel electrophoresis 111 25 AMB 1 experiment 25: Southern blot transfer 117 26 AMB 1 experiment 26: Probe labeling and purification 121 27 AMB 1 experiment 27: Dot blot assay 125 28 AMB 1 experiment 28: Pre-hybridization, hybridization and detection 127 29 AMB 1 experiment 29: Total yeast RNA isolation and RT-PCR 131 30 AMB 1 experiment 30: Yeast-based in vivo recombination cloning 139 31 AMB 1 experiment 31: Plasmid DNA isolation from yeast 143 32 AMB 1 experiment 32: E. coli transformation with yeast plasmid DNA 145 33 AMB 1 experiment 33: X-gal filter lift assay 147 34 AMB 1 experiment 34: Protein quantitation assay 149 35 AMB 1 experiment 35: Quantitative -Galactosidase assay in yeast 155 36 AMB 1 experiment 36: Gel filtration chromatography (GFC) 161 37 AMB 1 experiment 37: Ion exchange chromatography (IEC) 165 Part III: Advanced Methods in Biotechnology (AMB) 2 Laboratory Exercises 169 38 AMB 2 experiment 38: E. coli culture media preparation 171 39 AMB 2 experiment 39: Site-directed mutagenesis 175 40 AMB 2 experiment 40: Protein expression in E. coli 179 41 AMB 2 experiment 41: Protein purification by affinity column chromatography 187 42 AMB 2 experiment 42: SDS-PAGE analysis of affinity column fractions 193 43 AMB 2 experiment 43: Western blot analysis of affinity column fractions 199 44 AMB 2 experiment 44: Yeast media preparation and phenotypic analysis of yeast strains 203 45 AMB 2 experiment 45: Yeast transformation for yeast two-hybrid (Y2H) assay and genome editing by CRISPR-Cas system 209 46 AMB 2 experiment 46: Yeast mating-mediated Y2H assay and genomic PCR 215 47 AMB 2 experiment 47: Yeast colony PCR screening and cycle DNA sequencing 221 48 AMB 2 experiment 48: DNA sequencing electrophoresis 227 49 AMB 2 experiment 49: RNA interference 237 50 AMB 2 experiment 50: Protein preparation for 2D gel electrophoresis 241 51 AMB 2 experiment 51: Two-dimensional gel electrophoresis 245 Part IV: Appendices 253 1 Methods in Biotechnology Appendix 1 255 2 MB Appendix 2 261 3 MB Appendix 3 265 4 MB Appendix 4 267 5 AMB 1 Appendix 1 271 6 AMB 1 Appendix 2 281 7 AMB 1 Appendix 3 287 8 AMB 1 Appendix 4 291 9 AMB 1 Appendix 5 297 10 AMB 2 Appendix 1 299 11 AMB 2 Appendix 2 307 12 AMB 2 Appendix 3 313 13 AMB 2 Appendix 4 317 14 AMB 2 Appendix 5 319 15 AMB 2 Appendix 6 321 16 AMB 2 Appendix 7 325 Glossary 327 Abbreviations 335 Index 337
Copyright Date
2017
Topic
Biotechnology
Lccn
2016-499675
Dewey Decimal
660.6
Intended Audience
Scholarly & Professional
Dewey Edition
23
Illustrated
Yes
Genre
Science
Item description from the seller
Seller assumes all responsibility for this listing.
eBay item number:395295572078
Shipping and handling
Item location:
Calgary, Alberta, Canada
Ships to:
Worldwide
Excludes:
APO/FPO, Afghanistan, Alaska/Hawaii, Belarus, Dominica, Fiji, French Guiana, French Polynesia, Guadeloupe, Haiti, Honduras, Kuwait, Libya, Macedonia, Martinique, Morocco, Nicaragua, Papua New Guinea, Qatar, Russian Federation, Saudi Arabia, Tonga, Tuvalu, US Protectorates, Ukraine, United Kingdom, Vanuatu, Western Samoa, Yemen
| Shipping and handling | Each additional item | To | Service | Delivery*See Delivery notes |
|---|---|---|---|---|
| Free shipping | Free | United States | Economy Shipping | Estimated between Mon, Jun 3 and Tue, Jun 11 to 43230 |
| Handling time |
|---|
| Will ship within 10 business days of receiving cleared payment. The seller has specified an extended handling time for this item. |
| Taxes |
|---|
| Taxes may be applicable at checkout. Learn moreLearn more about paying tax on eBay purchases. |
Return policy
| After receiving the item, contact seller within | Refund will be given as | Return shipping |
|---|---|---|
| 30 days | Money Back | Buyer pays for return shipping |
Refer to eBay Return policyopens in a new tab or window for more details. You are covered by the eBay Money Back Guaranteeopens in a new tab or window if you receive an item that is not as described in the listing.
Payment details
Payment methods
Seller feedback (1,022,845)
i***6 (139)- Feedback left by buyer.
Past 6 months
Verified purchase
Item as described, good price, well packaged, arrived slightly later than hoped (not in time for Christmas ) but the order was placed in a very busy shipping period. No issue with the seller’s speed of response and sending the item. Great seller!
t***r (91)- Feedback left by buyer.
Past 6 months
Verified purchase
I was pleasantly surprised by how soon I received this textbook given that it shipped from outside the U.S. It was packaged well with no damage. The price was right. And I greatly appreciated the communication informing me where the book was shipping from and including a tracking number. Overall, a positive experience, - online shopping the way it should be. I've bought books from this seller several times now without any complaints and will surely buy from them again. Thanks a lot!
s***3 (147)- Feedback left by buyer.
Past month
Verified purchase
Quality and hard to find book that won't be released 'til January 2025 in the U.S.A.
Fantastic price.
Book was in a secure and protective package. Arrived a few days after I placed order and that was faster than I expected.
Seller is a fantastic communicator and proactive in regards to tracking status. I'd definitely buy from them in the future.
Product ratings and reviews
More to explore :
- Nonfiction Methodism Paperbacks Books,
- Paperbacks Books in English,
- Paperbacks Books in English,
- Trade Paperbacks Books in English,
- Trade Paperbacks Books in English,
- Fiction Trade Paperbacks Books in English,
- Paperback Dictionaries & Reference Books in English,
- Martial Arts Paperback Books in English,
- Nonfiction Trade Paperbacks Books in English,
- Suzanne Collins Paperbacks Books in English